pEXT01/H
Cat# exv01 pET28-His/N Vector Infomation
  
  5X EX-Buffer
EX-Vector
EXclonase Enzyme
Ship and Storage
40 μl
20 μl
10 μl
-20 oC

Vector pEXT01/H (pET28-His/N) is modified from plasmid pET28? which carry an N-terminal His tag, C-terminal His tag, and driven by T7 promoter.

   
Purpose:Express protein in Bacterial using pET system
Backbone:pET28
Promoter:T7
Size (bp):5999
N-ter Tag(s):His
C-ter Tag(s):His
Type:Bacterial Expression
5' primer:T7 promoter primer
3' primer:T7 terminator primer
Resistance(s):Kanamycin
Description
Vector pEXT01/H, short for pEXT01-His/N, is modified from plasmid pET28a which carry an N-terminal His?Tag / thrombin / T7?Tag configuration plus an optional C-terminal His?Tag sequence. This expression system provides the highest level of protein production available in any expression system. Expression is induced from the strong T7 lac promoter. This bacterial expression system is ideal for producing protein for bioproduction, antibody production, X-ray crystallography, or mass spectrometry analysis. Please refer to the pET expression system for further information.
Reference
pET expression system from Merck.
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